| 摘要: |
| ［摘要］目的 在博来霉素诱导肺纤维化小鼠肺组织中， 探讨IL-27是否通过TGF-β/Smad信号通路抑制肺纤维化．方法 将40只雄性C57/BL6小鼠随机分为：空白对照组（A组），博来霉素组（B组），博来霉素+白介素27组（C组），博来霉素+白介素27抗体组（D组），每组10只．于造模后第7、28天每组各处死5只小鼠，取右肺组织用Western Blot 的方法检测TGF-βR1、Smad1、Smad3蛋白的表达．结果（1）博来霉素诱导肺纤维化模型中，7 d、28 d肺组织均有TGF-βR1表达升高，IL-27治疗后降低TGF-βR1表达（P＜0.05）；（2）D组p-Smad1、p-Smad3表达量在造模后7 d、28 d均最高，C组在造模后7 d表达量少于B组（P＜0.05）．结论 外源性IL-27可能通过抑制TGF-β/Smad通路相关蛋白的磷酸化减轻肺纤维化． |
| 关键词: ［关键词］IL-27 肺纤维化 TGF-β/Smad信号通路 |
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| 基金项目:［基金项目］国家自然科学基金资助项目（ 81360015，81560015 ）；云南省应用基础研究基金资助项目（2013FB049）；云南省教育厅科研基金重点资助项目（2012Z082） |
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| IL-27 Alleviates the Bleomycin-induced Pulmonary Fibrosis through Regulating TGF-β/Smad Signaling Pathway |
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LEI Wen
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（Dept. of Respiratory Medicine，The 2nd Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650101，China）
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| Abstract: |
| ［Abstract］Objective To explore whether IL-27 inhibited the pulmonary fibrosis through regulating the expression of TGF-β/Smad signaling pathway in the bleomycin-induced pulmonary fibrosis model. Methods Forty male C57/BL6 mice were randomly divided into normal control group（group A）， bleomycin-induced pulmonary fibrosis group （group B）， bleomycin+IL-27 group（group C）and bleomycin+IL-27 antibody group （group D） with 10 in each. Five mice in each group were sacrificed on days 7 and 28 after with intratracheal bleomycin. TGF-βR1， Smad1 and Smad3 in right lung tissue were measured by Western Blot. Results 1. In the bleomycin-induced pulmonary fibrosis model， the expression of TGF-βR1 was higher on days 7 and 28， which was inhibited by IL-27. 2. The expressions of p-Smad1 and p-Smad3 were highest in group D on days 7 and 28， but were lower in group C on day 7 than those in group B. Conclusion Exogenous IL-27 might alleviate pulmonary fibrosis through inhibiting the related protein phosphorylation in TGF-β/Smad signaling pathway. |
| Key words: ［Key words］IL-27 Pulmonary fibrosisp TGF-β/Smad signaling pathway |