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乙型肝炎病毒在人类早期胚胎中的垂直传播
李永刚
(云南省第一人民医院生殖医学科,云南 昆明 650032)
摘要:
[摘要]目的 检测合并乙型肝炎病毒(HBV)感染的不孕不育症患者行体外受精-胚胎移植(IVF-ET)及衍生技术助孕后子代早期胚胎中HBV表达情况,为HBV经人类生殖细胞垂直传播子代提供可靠证据. 方法 收集在云南省第一人民医院生殖医学科行IVF-ET助孕的61对HBV感染不孕症夫妇患者废弃的早期胚胎,通过单细胞扩增技术获得全基因组DNA,采用聚合酶链式反应(PCR)技术检测HBV-DNA及HBV共价闭合环状DNA(HBV-cccDNA)在早期胚胎中的表达情况.结果 无论母方或父方或夫妻双方HBV感染患者的子代早期胚胎中均可检测到HBV-DNA和HBV-cccDNA表达阳性,且HBV-DNA在母方、父方和夫妻双方HBV感染患者的子代早期胚胎中的阳性检出率分别为78.9%、76.3%和100%,而HBV-cccDNA阳性检出率分别为63.2%、78.9%和100%,其中HBV-DNA和HBV-cccDNA阳性检出率在母方和父方合并HBV感染2组间均差异无统计学意义(P>0.05),但夫妻双方均合并HBV感染则阳性检出率显著增高(P<0.05). 结论 对于行IVF-ET助孕的HBV感染患者,HBV可通过母源性和父源性垂直传播至子代早期胚胎,且夫妻双方同时合并HBV感染的垂直传播风险显著增加,为HBV经人类生殖细胞垂直传播子代提供了可靠证据.
关键词:  [关键词]辅助生殖技术  乙型肝炎病毒  人类早期胚胎  垂直传播
DOI:
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基金项目:[基金项目]国家自然科学基金资助项目(81460234,81660247);云南省应用基础研究基金重点项目(2013FZ262);云南省对外科技合作计划项目-乔杰专家工作站基金资助项目(2016年)
Vertical Transmission of Hepatitis B Virus in Human Early Embryos
LI Yong-gang
(Dept. of Reproductive Medicine,The First People’s Hospital of Yunnan Province,Kunming Yunnan 650032,China)
Abstract:
[Abstract]Objective To detect the expression of HBV in human early embryos of infertility patients combined with hepatitis B virus(HBV)infection after undergoing in vitro fertilization and embryo transfer (IVF-ET) treatment,so as to provide reliable evidence for the vertical transmission of HBV through human germ cells. Methods We collected the abandoned early embryos of patients combined with HBV infection after undergoing IVF-ET treatment in the department of reproductive medicine of the First People's Hospital of Yunnan Province,total genomic DNA was obtained by single cell PCR,and then polymerase chain reaction(PCR)was used to detect the expression of HBV-DNA and covalently closed circular DNA of hepatitis B virus (HBV-cccDNA)in early embryos. Results Both HBV-DNA and HBV-cccDNA were detected in the early embryo among the mother or father or parents HBV infected patients. The positive detection rate of HBV-DNA in mother, father and parents HBV infected patients was 78.9%,76.3% and 100%,and the positive rate of HBV-cccDNA was 63.2%,78.9% and 100%. The difference between the mother and the father was not significant(P>0.05). However,the positive rate in parents HBV infected patients group was significantly higher than the mother or father group,showing a significant difference(P<0.05). Conclusion HBV can be transmitted from mother or father to early embryos via IVF-ET treament, and the risk of vertical transmission increases significantly when the couple are HBV infected.
Key words:  [Key words]Assisted reproductive technology  Hepatitis B virus  Human early embryos  Vertical transmission