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自身免疫性肝炎C57BL/6小鼠肝脏病理特点的实验研究
张志波1,2,3
1.(1)昆明市延安医院消化内科,云南 昆明 650051;2) 昆明市官渡区吴井街道社区卫生服务中心,云南 昆明 650200;3)昆明医科大学基础医学院,云南 昆明 650500)
摘要:
[摘要]目的 观察自身免疫性肝炎(AIH)C57BL/6小鼠的肝脏病理特点.方法 以等体积同种系小鼠肝抗原和完全弗氏佐剂乳化混合为免疫剂,经腹腔注射给C57BL/6小鼠建立AIH C57BL/6小鼠模型.检测正常对照组(N组)和AIH模型组(M组)小鼠体重、丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)的变化;比较两者肝脏、脾脏、心脏、肺脏、肾脏组织的病理特点.结果 成功建立了AIH C57BL/6小鼠模型.M组小鼠平均体重增长幅度较N组小鼠明显降低,M组小鼠血清ALT和AST升高较N组小鼠明显.与N组小鼠相比,M组小鼠肝脏呈现典型的界面性肝炎、淋巴细胞浸润,成重症肝炎表现;出现肝细胞灶状坏死,甚至多小叶坏死;有纤维结缔组织增生,出现肝纤维化的早期表现.M组小鼠脾脏明显肿大,色泽变深,边缘不齐,表明不光滑.差异有统计学意义(P<0.05).而2组小鼠在肺脏、心脏和肾脏之间病理特点比较未见明显异常,差异无统计学意义(P>0.05).结论 AIH C57BL/6模型小鼠肝脏病理特点与AIH患者相似,为自身免疫性肝炎的实验研究提供了病理依据.
关键词:  [关键词]自身免疫性肝炎  C57BL/6小鼠  肝脏  病理
DOI:
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基金项目:[基金项目]昆明市卫生科技人才培养项目[SW(后备)-01];昆明市延安医院院内课题(yyky012-34)
Experimental Research on Liver Pathological Characteristics of Autoimmune Hepatitis C57BL/6 Mice
ZHANG Zhi-bo1,2,3
1.(1)The Affiliated Yan’an Hospitpl of Kunming Medical University,Yunnan Kunming 650051;2)Community Health Service Center of Wu Jing Street,Guandu District,Kunming,Yunnan Kunming 650200;3)School of Basic Medicine,Kunming Medical University,Yunnan Kunming 650500,China)
Abstract:
[Abstract]Objective To observe liver pathological characteristics of the autoimmune hepatitis (AIH) C57BL/6 mice.Methods We established AIH C57BL/6 mice model with mixed adjuvants of allogenic liver antigens and complete freund's adjuvant by intraperitoneal injection. Then we determined and compared the body weight, alanine aminotransferase(ALT),aspartate aminotransferase(AST) between the normal control group (N group) and AIH model group(M group). And we compared the pathological characteristics of liver,spleen, heart, lung, kidney between two groups. Results We successfully established AIH C57BL/6 mice model. Compared with N group, we found that the average weight increase of mice in M group was decreased,ALT and AST of mice in M group were boosted. In M group, liver of mice presented typical interface of hepatitis, lymphocytic infiltration,even severe hepatitis,and showed spotty necrosis, multi-acinar necrosis. Some showed early sign of liver fibrosis by aprearing fibrous tissue hyperplasia. M group mouse's spleens were enlarged significantly. The spleen had darker color, not neat, and not smooth. Meanwhile, results were analyzed with statistics to confirm whether there was a significant difference between two groups(P<0.05). About lungs, hearts and kidneys of mice in two groups,the pathological features were not found, and there was no statistic difference (P>0.05). Conclusions The liver pathological characteristics of AIH C57BL/6 mice are similar to AIH patients. The results provide the pathological basis for the experimental research of autoimmune hepatitis.
Key words:  [Key words]Autoimmune hepatitis  C57BL/6 mice  Liver  Pathology