| 摘要: |
| ［摘要］目的 研究慢性牙周炎患者牙袋内厌氧菌的分布及对抗生素的耐药情况，为临床慢性牙周炎患者的抗感染治疗提供参考依据．方法 以84例牙周炎患者为研究对象，对患者病变牙袋内的厌氧菌进行分离培养和鉴定，并用琼脂稀释法检测所分离的厌氧菌对甲硝唑、青霉素、头孢美唑等7种抗生素的敏感性．结果 从84例慢性牙周炎患者的牙周袋内共分离出305株可疑致病菌，所有患者均检出厌氧菌感染．检出菌中革兰氏阴性菌占59.34%，革兰氏阳性菌占40.66%．产黑色素雷沃菌和牙龈卟啉单胞菌的检出率最高，分别为51.19%和55.95%，其次为中间普雷沃菌（36.90%），微小消化链球菌（35.71%），具核梭杆菌（33.33%），二氧化碳嗜纤维菌（27.38%），放线菌（10.71%）等．药敏结果显示，所检出菌株对氯霉素和亚胺培南全部敏感，对阿莫西林敏感率为98.03%，对头孢美唑和甲硝唑的敏感率在76%～88%之间，而对克林霉素和青霉素的敏感度最低为65.91%和55.73%．结论 厌氧菌感染与慢性牙周炎的发生密切相关，产黑色素雷沃菌和牙龈卟啉单胞菌是其中的优势菌，分离菌对氯霉素和亚胺培南的敏感度较高，两者可作为治疗慢性牙周炎的辅助用药． |
| 关键词: ［关键词］慢性牙周炎 厌氧菌 药敏实验 |
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| 基金项目:［基金项目］卫生部部属医院临床重点学科基金资助项目［卫规财函2010（439）］ |
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| Distribution and Antimicrobial Susceptibility of Anaerobic Bacteria in Periodontal Pocket of 84 Patients with Chronic Periodontitis |
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MA Jin-hua1,2
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1.（1）Dental Department，Central Hospital of Suining City，Sichuan Province，Suining Sichuan 629000;2）Oral and Maxillofacial Surgery Department，Oral subsidiary Sun Yat-sen University Hospital，Guangzhou Guangdong 510000，China）
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| Abstract: |
| ［Abstract］Objective To study the distribution and antimicrobial susceptibilities of anaerobic bacteria from periodontal pockets of patients with chronic periodontitis， so as to guide the anti-infection therapy. Methods Eighty-four patients with chronic periodontitis were enrolled in this study. The anaerobes were isolated from periodontal pockets of patients with chronic periodontitis. Antimicrobial susceptibility of the anaerobes to seven kinds of antibiotics （like metronidazole， penicillin， cefmetazole， etc.） was tested with the agar dilution technique. Results A total of 305 cultivable anaerobes were isolated from periodontal pockets of 84 patients with chronic periodontitis. All patients were infected by anaerobes. 59.34% of cultivable anaerobes were gram-positive anaerobes，and 40.66% were gram-negative anaerobes. The P.melanin ogenica and P.gingivalis were the most common among the cultivable anaerobes， accounting for 51.19% and 55.95%， followed by P.intermedia （36.90%）， P.micros（35.71%），F.mucleatum （33.33%）， Capnocytophaga ssp（27.38%），Actinomyces ssp （10.71%）， and so on. Most isolated anaerobes were sensitive to seven antibiotics. All isolated anaerobes were susceptible to Chloromycetin and Imipenem. 98.03% of isolated anaerobes were susceptible to Amoxicillin. Approximately 76% to 88% of isolated anaerobes were susceptible to Cefmetazole and Metronidazole. Susceptibility to Clindamycinum and Penicillin were 65.91% and 55.73%. Conclusions There is a close relationship between chronic periodontitis and anaerobes. P.melanin ogenica and P.gingivalis are the most frequently isolated anaerobes from periodontal pockets of patients with chronic periodontitis. Chloromycetin and Imipenem can be used for the treatment of anaerobes infected chronic periodontitis. |
| Key words: ［Key words］Chronic periodontitis Anaerobic bacteria Microbial sensitivity tests |