| 摘要: |
| ［摘要］目的 研究老鹳草素对骨质疏松（OP）大鼠骨髓基质干细胞（BMSCs）信号分子Wnt3a表达的影响．方法 摘除大鼠双侧卵巢，复制骨质疏松症模型，分离、培养BMSC．将源于假手术大鼠第3代BMSCs常规培养作为正常对照组，将骨质疏松模型大鼠的BMSCs分别设以下组别：模型对照组、1 μmol/L的辛伐他汀阳性对照组、0.01、0.1、1、10、100 μmol/L的老鹳草素组．分别采用实时荧光定量PCR和Western blot方法检测mRNA及蛋白的表达．结果 与正常对照组比较，OP模型对照组Wnt3a蛋白及其mRNA的表达明显降低，且有统计学意义（P＜0.01）；与模型组比较，1 μmol /L辛伐他汀、0.1、1、10、100 μmol/L的老鹳草素明显升高Wnt3a蛋白及其mRNA的表达，且均有统计学意义，而0.01 μmol/L老鹳草素对Wnt3a表达无明显影响．结论 老鹳草素可能通过增加BMSC中Wnt/β-catenin信号通路的信号蛋白Wnt3a的表达，进一步激活Wnt/β-catenin信号通路，这有利于BMSC的成骨分化及骨形成． |
| 关键词: ［关键词］老鹳草素 骨质疏松 骨髓基质干细胞 Wnt3a |
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| 基金项目:［基金项目］国家自然科学基金资助项目（30660212、81160401、81260493）；云南省社会发展计划重点项目（2008CC009）；云南省科技厅-昆明医科大学联合专项基金资助项目（2013FB103） |
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| Effect of Geraniin on Expression of Wnt3a in BMSC from Osteoporotic Rats |
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LI Li1,2
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1.（1）Dept. of Pharmacy，The 3rd Afiliated Hospital of Kunming Medical University，Kunming Yunnan 650118;2）Pharmaceutical College & Key Laboratory of Pharmacology for Natural Products of Yunnan Province，Kunming Medical University，Kunming Yunnan 650500，China）
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| Abstract: |
| ［Abstract］Objective To investigate the effect of geraniin on expression of Wnt3a protein and mRNA in bone marrow stromal cell （BMSC） from osteoporotic rats. Methods The model of osteoporosis （OP） was duplicated by ovariectomy in rats. BMSCs were isolated and cultured. BMSCs from shamed rats were routinly cultured and taken as normal control， and BMSCs from OP rats were divided into model group， 1 μmol/L simvastatin positive group， and geraniin group （0.01， 0.1， 1， 10， 100 μmol/L）， respectively. The methods of realtime-PCR and western blot were used to assay the protein and mRNA expression of wnt3a， respectively．Results As compared with normal control group， the protein and mRNA expression of wnt3a in model group were significantly suppressed; Compared with model group， 1 μmol/L simvastatin， and 0.1， 1， 10 and 100 μmol/L geraniin significantly increased the expression of wnt3a protein and mRNA. Conclusion It is suggested that geraniin activates wnt/β-catenin pathway though increasing the expression of signaling protein wnt 3a in BMSCs from OP rats. It would be beneficial to osteogenic differentiation of BMSCs and osteogenesis. |
| Key words: ［Key words］Geraniin Osteoporosis Bone marrow stromal cells Wnt3a |