| 摘要: |
| ［摘要］目的 发掘与肺癌相关的特异性miRNA用于肺癌的分型分期和预后判断．方法 对47例肺癌患者手术切除的肺癌组织和癌旁正常肺组织用SYBR Green实时荧光定量PCR对miRNA-192进行定量检测，比较肺癌组织和癌旁正常肺组织miRNA-192的表达是否有差异．结果 吸烟者，每天吸烟支数＞3支者，吸烟年数＞2 a及饮酒者的肺癌患者肺癌组织miRNA-192表达量较不吸烟、每天吸烟支数＜3支者，吸烟年数＜2 a及不饮酒者高．总的47个病例miRNA-192在肺癌组织中表达量△CT值为（8.19±2.94），正常肺组织的表达量ΔCT值为（7.42±2.64），经配对t 检验，P＞0.05 ，miRNA-192在肺癌组织中的表达与正常肺组织差异无统计学意义（P＞0.05）；经相关分析肺癌组织中的miRNA-192表达倍数与临床资料之间无相关关系．结论 miRNA-192在具有吸烟及饮酒危险因素的人群中表达增高，可认为miRNA-192在肺癌的发生中具有一定的作用，但因总的47个病例的miRNA-192在肺癌组织和正常肺组织中表达差异无统计学意义，因此还无法确定miRNA-192是否属于肺癌的癌基因或抑癌基因，miRNA-192目前还不能用于肺癌的分型分期和预后判断． |
| 关键词: ［关键词］肺癌 MicroRNA-192 实时荧光定量PCR |
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| 基金项目:［基金项目］云南省应用基础研究基金资助项目 （2013FZ063）；云南省教育厅科学研究基金重点资助项目（2012Z077） |
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| Expression and Clinical Significance of miRNA-192 in Lung Cancer Tissues |
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MA Qin－xin1,2,3
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1.（1）School of Public Health，Kunming Medical University，Kunming Yunnan 650500;2.２）Dept. of Gastroenterology，The 2nd Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650101;3.３）Dept. of Cardiac and Thoracic Surgery，The 3rd Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650118，China）
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| Abstract: |
| ［Abstract］Objective To explore the lung cancer related specific miRNA for judging the pathological type and disease progression．Methods The 47 samples were got by multi-stage randomized sampling. TRIzol was used to extract the total of RNA from tissue samples，real-time SYBR Green fluorescent quantitative polymerase chain reaction（PCR）was used for quantitative detection of miRNA-192. The experimental data were statistically analyzed by the SPSS software．Results The expression levels of miRNA-192 in lung cancer tissues of patients severe smoking（over 3 cigrattes one day for more than 2 years ）and drinking were significantly higher than that in patients without smoking and drinking or mild smoking（less than 3 cigrattes one day or less than 2 years）. In the 47 cases， the expression of miRNA-192 had no significant difference in lung cancer tissues （△CT=8.19±2.94） and in normal lung tissues（7.42±2.64）（P＞0.05）. There was no correlation between the expression of miRNA-192 in lung cancer tissues and the clinical data．Conclusions MiRNA-192 is up-regulated in the population with risk factors such as smoking and drinking， and plays an role in the pathogenesis of lung cancer.There was no significant difference in miRNA-192 expression between lung cancer tissues and normal lung tissues，so we can't make sure miRNA-192 as a oncogene or suppressor gene of lung cancer. miRNA-192 can't be used in judging the pathological types，staging and prognosis of lung cancer. |
| Key words: ［Key words］Lung cancer MicroRNA （MiRNA）-192 Real-time PCR |