| 摘要: |
| ［摘要］目的 检测新纤维化相关因子SFTPA2在大鼠肾间质纤维化模型中的表达变化．方法 64只SD大鼠，雌雄各半，随机分为单侧输尿管梗阻模型组（UUO）、假手术组、siRNA组和阴性对照组，分别于术后3 d、5 d、7 d、14 d分批（n＝4）处死，留取手术侧（模型组梗阻侧）肾脏组织，采用HE染色评价肾小管间质纤维化损伤；采用ELISA法检测大鼠血SFTPA2、转化生长因子（TGF-β1）、平滑肌肌蛋白（α-SMA）、血管内皮生长因子（VEGF）的表达；采用western blot法检测肾皮质SFTPA2、E-cadherin、平滑肌肌动蛋白（α-SMA）蛋白表达水平．结果 肾脏组织形态学观察显示，与假手术组相比，随着输尿管梗阻时间的延长，肾小管间质纤维化程度逐步加重．ELISA实验结果表明，与假手术组相比，模型组大鼠血液中TGF-β1 、SFTPA2的表达随着梗阻时间延长逐渐升高，7 d起明显升高（P＜0.05），于14 d达高峰；与阴性对照组相比，siRNA组大鼠血液中TGF-β1 的表达于7 d开始升高，14 d开始下降（P＜0.05），而SFTPA2的表达于第5天开始升高，14 d开始下降（P＜0.05）．Western实验结果表明，模型组中SFTPA2的表达在术后7 d开始上升，于14 d达高峰；siRNA组中SFTPA2的表达在术后3 d开始上升，于7 d达高峰，14 d开始下降．但TGF-β1 和SFTPA2未表现出明显的相关性． 结论 在大鼠肾间质纤维化模型中，大鼠血液及肾组织中SFTPA2的表达增加，提示SFTPA2与肾纤维化相关；TGF-β1 与SFTPA2无明显相关性． |
| 关键词: ［关键词］肾小管间质纤维化 单侧输尿管梗阻 SFTPA2 转化生长因子β1 |
| DOI： |
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| 基金项目:［基金项目］云南省应用基础研究基金资助项目（2012FB029）；云南省教育厅科学研究基金资助项目（2014J40） |
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| Expression and Effects of New Fibrotic Gene SFTPA2 in Unilateral Ureteral Obstruction Rats with Renal Interstitial Fibrosis |
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LI Ye
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（Dept. of Nephrology，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China）
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| Abstract: |
| ［Abstract］Objective To detect the changs in expression of new fibrosis gene SFTPA2 in rat with renal interstitial fibrosis. Methods Sixty-four SD rats，half male and female,were randomly divided into UUO group，sham-operation group，SiRNA group，negative control group，rats were sacrified 3,5,7,14d after operation （n=4 for each day），and the renal tissues of the operated side（obstructed side for model group）were obtained. HE staining was used to evalute the degree of renal tubulointerstitial fibrosis. ELISA was used to detect the expression of SFTPA2，transforming growth factor-β1 （TGF-β1）α-smooth musdeactin（α-SMA），vascular endothelial grouth factor（VEGF）of blood. Western blot was used to detect the expression of SFTPA2，E-cadherin，α-smooth muscle actin（α-SMA）in renal tissues. Results Histological observations of renal tissue revealed that compared with sham operation group，the degree of renal tubulointerstitial fibrosis gradully increased with time of ureteral obstruction. ELISA showed that compared with sham operation group,the expression of TGF-β1 and SFTPA2 in UUO group increased with time of ureteral obstruction，especially till 7d（P＜0.05），top till 14 d. Compared with negative control group,the expression of TGF-β1 increased till 7d，decreased till 14d（P＜0.05），the expression of SFTPA2 increased till 5d，decreased till 14d（P＜0.05）.Western blot showed that the expression of SFTPA2 increased till 7 d，top till 14d in UUO group；the expression of SFTPA2 increased till 3d，top till 7 d dreased till 14 d，but there was no obvious relationship between them. Conclusion The progression of increase in expression of SFTPA2 in blood and renal tissues of rats may be related to renal interstitial fibrosis. But there is no obvious relationship between SFTPA2 and TGF-β1. |
| Key words: ［Key words］Renal tubulointerstitial fibrosis Unilateral ureteral obstruction SFTPA2 TGF-β1 |