| 摘要: |
| ［摘要］目的 探讨枫叶黄酮对脂多糖 （lipopolysaccharide，LPS）诱导破骨前体细胞Raw264.7细胞炎性因子释放的抑制作用．方法 用LPS （5 mg/mL）刺激Raw264.7细胞构建炎症模型，采用四甲基偶氮唑蓝比色法 （MTT）检测Raw264.7细胞的活力影响；酶联免疫吸附试验（ELISA）检测一氧化氮 （NO）和前列腺素E2 ， PGE2表达；运用免疫荧光染色方法检测诱导型一氧化氮合酶和环氧合酶-2，COX-2、子肿瘤坏死因子-αTNF-α和白介素-1β，IL-1β、核因子-κB，NF-κB蛋白与mRNA的表达变化． 结果 不同浓度的枫叶黄酮明显抑制了LPS诱导的Row246.7细胞NO、PGE2，iNOS和COX-2，TNF-α和IL-1β与NF-κB的上调，并且NO、PGE2、iNOS、COX-2和NF-κB蛋白的表达呈剂量依赖性． 结论 枫叶黄酮可抑制LPS诱导的破骨前体细胞Raw264.7细胞iNOS、COX-2和NF-κB/P65蛋白表达和炎性因子释放从而抑制破骨细胞的激活 |
| 关键词: ［关键词］枫叶黄酮 脂多糖 炎性因子 Raw264.7细胞 免疫荧光染色 |
| DOI： |
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| Flavonoid Isolated from the Leaves of Acer Truncatum Bunge Inhibits Lipopolysaccharide-induced Activation of Raw264.7 Cells |
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ZOU Zhi－rong
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Dept. of Anatomy，Kunming Medical University
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| Abstract: |
| ［Abstract］Objective To investigate the inhibitory effects of flavonoid isolated from the leaves of Acer truncatum Bunge on the expression of pro-inflammatory cytokines in Raw264.7 cells stimulated with lipopolysaccharide （LPS）．Methods Inflammatory cell model was structured by LPS-stimulated Raw264.7 cells，the cells were treated with flavonoid（10 μM， 30 μM and 60 μM） prior to LPS（5 mg/mL）exposure and the effects of flavonoid on viability of Raw264.7 cells were measured by MTT assay，Nitric oxide（NO）and prostaglandin E2 （PGE2）were measured by ELISA，the effects on the protein levels of pro-inflammatory enzymes，cyclooxygenase-2（COX-2）and inducible nitric oxide synthase（iNOS），and pro-inflammatory cytokines，tumor necrosis factor-α（TNF-α），and interleukin-1β（IL-1β），inflammatory signaling proteins nuclear factor-κB （NF-κB）were analysed by immunofl uorescence staining assay．Results LPS-induced NO，PGE2，iNOS， COX-2 and NF-κB protein expression levels were significantly decreased by flavonoid in a concentration-dependent manner and flavonoid also had an effect on the expression of TNF-α，IL-1β through translational inhibition． Conclusion Flavonoid isolated from the leaves of Acer truncatum Bunge can inhibit LPS-induced activation of Raw264.7 cells through inhibiting the release of pro-infl ammatory mediators，then exerts its anti-osteoporosis activities. |
| Key words: ［Key words］Flavonoid Lipopolysaccharide Pro-inflammatory cytokines Raw264.7 cells Immuno uorescence staining |