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人脐带间充质干细胞对再生障碍性贫血患者T细胞相关因子调节的体外研究
邰文琳,张丹丹,王殿华
1. 昆明医学院第二附属医院检验科,云南,昆明,650101
2. 昆明医学院药学院暨云南省天然药物药理重点实验室,云南,昆明,650100
摘要:
[摘要]目的 体外观察人脐带间充质干细胞对再生障碍性贫血患者和正常对照T细胞相关细胞因子IL-2、IFN-γ、IL-17、IL-6的调节作用.方法 采用组织块培养法从脐带分离、培养间充质干细胞.用流式细胞术鉴定脐带间充质干细胞的表型.分离再生障碍性贫血患者和正常对照外周血单个核细胞与脐带间充质干细胞共培养.用ELISA法测定外周血单个核细胞组和共培养组培养上清中细胞因子IL-2、IFN-γ、IL-17和IL-6的水平.结果 脐带来源间充质干细胞高表达CD29、CD44、CD105、HLA-I.人脐带间充质干细胞与再生障碍性贫血患者外周血单个核细胞共培养后上清中IL-2水平无明显变化 (P>0.05)而IFN-γ水平有所下降(P>0.05),IL-17水平和IL-6水平明显上升(P<0.01).人脐带间充质干细胞与正常对照外周血单个核细胞共培养后上清中IL-2和IFN-γ水平明显下降(P<0.05)而IL-17水平无明显变化(P>0.05),IL-6水平明显上升(P<0.01).结论 人脐带间充质干细胞明显可抑制正常外周血单个核细胞分泌IL-2、IFN-γ,其与外周血单个核细胞共培养后可升高IL-6水平
关键词:  [关键词]再生障碍性贫血  脐带间充质干细胞  T 细胞
DOI:
分类号:R329.2+8
基金项目:
The Research of Mouse Bone Marrow-derived Mesenchymal Stem Cells Culture Condition in vitro
TAI Wen-lin,ZHANG Dan-dan,WANG Dian-hua
TAI Wen-lin1),ZHANG Dan-dan2),WANG Dian-hua2)(1)Dept.of Clinical Laboratory,The 2nd Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650101,2)School of Pharmacy and Yunnan Key Laboratory of Pharmacology for Natural Products,Kunming Medical University,Kunming Yunnan 650031,China)
Abstract:
Objective To research the whole bone marrow adherence culure condition of mouse bone marrow-derived mesenchymal stem cells,select the best condition,and establish an expansion method of mouse bone marrow-derived mesenchymal stem cells in vitro.Methods We detected the proliferation ability of the bone marrow mesenchymal stem cells in different ages mouse,different concentration of serum and cell density,and found out the optimum culture condition.BMSCs were cultured in the optimum culture condition,and light...
Key words:  Marrow-derived mesenchymal stem cells  The whole bone marrow adherence culture method  Research